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<t>EphA2,</t> ephrin-A1, EphB4, and ephrin-B1 localization in LSECs and Kupffer cells. Immunofluorescence micrographs showing EphA2, ephrin-A1, EphB4, and ephrin-B1 immunoreactivity (red) in CD146-positive LSECs (green) and F4/80-positive Kupffer cells (green) in the mouse liver. Cryostat sections were stained with the indicated antibodies and DAPI (blue). Green fluorescence images were merged with red, and/or blue fluorescence images in the same field. EphA2, ephrin-A1, EphB4, and ephrin-B1 immunoreactivity was localized in CD146-positive LSECs and weakly localized in F4/80-positive Kupffer cells (arrows). M, marginal region of the hepatic lobules; Pc, pericentral region of the hepatic lobules.
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<t>EphA2,</t> ephrin-A1, EphB4, and ephrin-B1 localization in LSECs and Kupffer cells. Immunofluorescence micrographs showing EphA2, ephrin-A1, EphB4, and ephrin-B1 immunoreactivity (red) in CD146-positive LSECs (green) and F4/80-positive Kupffer cells (green) in the mouse liver. Cryostat sections were stained with the indicated antibodies and DAPI (blue). Green fluorescence images were merged with red, and/or blue fluorescence images in the same field. EphA2, ephrin-A1, EphB4, and ephrin-B1 immunoreactivity was localized in CD146-positive LSECs and weakly localized in F4/80-positive Kupffer cells (arrows). M, marginal region of the hepatic lobules; Pc, pericentral region of the hepatic lobules.
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<t>EphA2,</t> ephrin-A1, EphB4, and ephrin-B1 localization in LSECs and Kupffer cells. Immunofluorescence micrographs showing EphA2, ephrin-A1, EphB4, and ephrin-B1 immunoreactivity (red) in CD146-positive LSECs (green) and F4/80-positive Kupffer cells (green) in the mouse liver. Cryostat sections were stained with the indicated antibodies and DAPI (blue). Green fluorescence images were merged with red, and/or blue fluorescence images in the same field. EphA2, ephrin-A1, EphB4, and ephrin-B1 immunoreactivity was localized in CD146-positive LSECs and weakly localized in F4/80-positive Kupffer cells (arrows). M, marginal region of the hepatic lobules; Pc, pericentral region of the hepatic lobules.
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Image Search Results


EphA2, ephrin-A1, EphB4, and ephrin-B1 localization in LSECs and Kupffer cells. Immunofluorescence micrographs showing EphA2, ephrin-A1, EphB4, and ephrin-B1 immunoreactivity (red) in CD146-positive LSECs (green) and F4/80-positive Kupffer cells (green) in the mouse liver. Cryostat sections were stained with the indicated antibodies and DAPI (blue). Green fluorescence images were merged with red, and/or blue fluorescence images in the same field. EphA2, ephrin-A1, EphB4, and ephrin-B1 immunoreactivity was localized in CD146-positive LSECs and weakly localized in F4/80-positive Kupffer cells (arrows). M, marginal region of the hepatic lobules; Pc, pericentral region of the hepatic lobules.

Journal: Biomedicines

Article Title: Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells

doi: 10.3390/biomedicines10123234

Figure Lengend Snippet: EphA2, ephrin-A1, EphB4, and ephrin-B1 localization in LSECs and Kupffer cells. Immunofluorescence micrographs showing EphA2, ephrin-A1, EphB4, and ephrin-B1 immunoreactivity (red) in CD146-positive LSECs (green) and F4/80-positive Kupffer cells (green) in the mouse liver. Cryostat sections were stained with the indicated antibodies and DAPI (blue). Green fluorescence images were merged with red, and/or blue fluorescence images in the same field. EphA2, ephrin-A1, EphB4, and ephrin-B1 immunoreactivity was localized in CD146-positive LSECs and weakly localized in F4/80-positive Kupffer cells (arrows). M, marginal region of the hepatic lobules; Pc, pericentral region of the hepatic lobules.

Article Snippet: Goat polyclonal antibodies against EphA2 (AF639), EphB4 (AF446), and ephrin-B1 (AF473) (R&D Systems, Minneapolis, MN, USA) and rabbit polyclonal antibody against ephrin-A1 (SAB4500696; Sigma-Aldrich) were used.

Techniques: Immunofluorescence, Staining, Fluorescence

Expression of Ephs and ephrins in the mouse liver, liver Mø propagated using mixed culture, and LSECs. ( A ) The protein expression and tyrosine phosphorylation (PTyr) of EphA2 and EphB4 were detected using Western blotting in the mouse liver. EphA2 and EphB4 in the liver are highly and weakly tyrosine-phosphorylated, respectively. IP, immunoprecipitation. ( B , C ) RT-PCR analysis of all Ephs and ephrins in liver Mø propagated using mixed culture ( B ) and primary LSECs ( C ). Liver Mø express Epha2 , Epha4 , Efna1 , Efna4 , Efna5, Ephb3 , Ephb4 , Ephb6 , Efnb1 , Efnb2 , and Efnb3 whereas LSECs express Epha2 , Efna1 , Ephb4 , Efnb1 , Efnb2 , and Efnb3 .

Journal: Biomedicines

Article Title: Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells

doi: 10.3390/biomedicines10123234

Figure Lengend Snippet: Expression of Ephs and ephrins in the mouse liver, liver Mø propagated using mixed culture, and LSECs. ( A ) The protein expression and tyrosine phosphorylation (PTyr) of EphA2 and EphB4 were detected using Western blotting in the mouse liver. EphA2 and EphB4 in the liver are highly and weakly tyrosine-phosphorylated, respectively. IP, immunoprecipitation. ( B , C ) RT-PCR analysis of all Ephs and ephrins in liver Mø propagated using mixed culture ( B ) and primary LSECs ( C ). Liver Mø express Epha2 , Epha4 , Efna1 , Efna4 , Efna5, Ephb3 , Ephb4 , Ephb6 , Efnb1 , Efnb2 , and Efnb3 whereas LSECs express Epha2 , Efna1 , Ephb4 , Efnb1 , Efnb2 , and Efnb3 .

Article Snippet: Goat polyclonal antibodies against EphA2 (AF639), EphB4 (AF446), and ephrin-B1 (AF473) (R&D Systems, Minneapolis, MN, USA) and rabbit polyclonal antibody against ephrin-A1 (SAB4500696; Sigma-Aldrich) were used.

Techniques: Expressing, Phospho-proteomics, Western Blot, Immunoprecipitation, Reverse Transcription Polymerase Chain Reaction